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Image Search Results
Journal: PLoS ONE
Article Title: Abl Family Kinases Regulate Endothelial Barrier Function In Vitro and in Mice
doi: 10.1371/journal.pone.0085231
Figure Lengend Snippet: ( A ) Staining of HMVEC monolayers for the adherens junction marker VE-cadherin (green) following treatment with VEGF (100ng/mL, 30 minutes), with or without imatinib pre-treatment (10μM). ( B ) VE-cadherin staining (red) of HMVEC monolayers treated with thrombin (1U/mL, 5 minutes), +/- imatinib. ( C ) VE-cadherin staining (red) of VEGF or thrombin-treated HMVECs, with or without GNF-2 pre-treatment (15μM). VEGF and thrombin treatment induced formation of inter-endothelial cell gaps (arrows) and destabilization of endothelial cell-cell junctions (“zig-zag” VE-cadherin staining pattern, arrowheads), which were reduced by pre-treatment with Abl kinase inhibitors.
Article Snippet: Antibodies used for both Western blotting and immunoprecipitation included
Techniques: Staining, Marker